Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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The Use of FDA to Assess the Viability of Preimplantation Mouse Embryo In vitro

생쥐배의 생존성 평가에 있어 FDA의 이용

  • Kim, Jae-Myeoung (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Hong, Jin-Ki (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Suh, Byung-Hee (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Lee, Jae-Hyun (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Chung, Kil-Sheoung (College of Animal Husbandary, Kon Kuk University)
  • 김재명 (경희대학교 의과대학 산부인과학교실(불임크리닉)) ;
  • 홍진기 (경희대학교 의과대학 산부인과학교실(불임크리닉)) ;
  • 서병희 (경희대학교 의과대학 산부인과학교실(불임크리닉)) ;
  • 이재현 (경희대학교 의과대학 산부인과학교실(불임크리닉)) ;
  • 정길생 (건국대학교 축산대학 축산학과)
  • Published : 1992.06.30
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Abstract

A fluorescence microscopy technique using flurescein diacetateCFDA) as a substract has been tested for the evaluation of the viability of early mouse embryos. Embryos were incubated in T6 containing FDA concentrations of 2.5 to $50{\mu}g/ml$ for 1 to 5min. Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Nicon Diaphot microscopy. The results were as follow. 1. The rate of fluorescein accumulation increased on the concentration on FDA from $2.5{\times}10^{-6}M$ to $20{\times}10^{-6}M$ 2. The rate at which intracellular fluorescein was lost from embryos was depended on the temperature at which are stored. 3. Embryos with 3 min exposure to FDA have the most intensity of fluorescence. 4. Exposure of 2 cell embryos to FDA ($2.5-5{\mu}g/ml$) for 1 min did not alter their ability to delope normally in vitro.

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