Korean Journal of Veterinary Research (대한수의학회지)
- Volume 32 Issue 3
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- Pages.429-434
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- 1992
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Optimization of liquid phase enzyme immunoassay for determining of progesterone
Progesterone 측정을 위한 액상(液相) 효소면역측정법(酵素免疫測定法)의 최적조건에 관한 연구
- Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University) ;
- Choi, Il-kwan (College of Veterinary Medicine, Gyeongsang National University) ;
- Son, Min-soo (College of Veterinary Medicine, Gyeongsang National University) ;
- Hur, Ju-hyeong (College of Veterinary Medicine, Gyeongsang National University) ;
- Kim, Chur-ho (Gyeongsangnam Do Veterinary Senice Laboratory)
- Received : 1991.12.07
- Published : 1992.07.30
Abstract
This study was carried out to develop an effective liquid-phase double antibody enzyme immunoassay for determining of progesterone. The optimum conditions of assay system, 1st and 2nd antibodies, enzyme conjugate, and time reaction were invested. The bovine plasma progesterone level in dairy cattle and korean native bulls were also analyzed. The results obtained were as follows; 1. The reproducibility of petroleum ether was superior to that of ethyl ether as extract solvent of progesterone in plasma. 2. The optimum dilution rate of 1st and 2nd antibody was 30,000 and 10 times, respectively. Affer the reaction of enzyme conjugate to progesterone 1st antibody, and then 2nd antibody competition reaction was enough for over 1hr. 3. Average plasma progesterone level in 4 pregnant and 9 nonpregnant Holstein was