Korean Journal of Veterinary Research (대한수의학회지)
- Volume 32 Issue 1
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- Pages.135-142
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- 1992
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
In vitro maturation of porcine follicular oocytes
돼지 난포내 난모세포의 체외성숙에 관하여
- Park, Mi-hee (Gyeongnam Veterinary service Laboratory) ;
- Lee, Hyo-jong (College of Veterinary Medicine, Gyeongsang National University)
- Received : 1991.09.05
- Published : 1992.01.30
Abstract
This experiment was carried out to establish an effective technique of in vitro maturation of porcine follicular oocytes. Porcine ovaries were collected from an abbatoir and delivered to the laboratory in phosphate buffered saline in an hour. Immatured follicular oocytes were collected from the ovaries and divided into groups by the size of follicles and by the attachment of granulosa cells. The follicular oocytes were cultured in m-KRB solution supplemented with FCS(10%), follicular fluid(10%) or hormones of PMSG(10IU/ml), hCG(10IU/ml ) and