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Studies on the Glycolipid Biosurfactant(2);The Rhamnolipid Production and Isolation by Pseudomonas sp. 13

당 지질계 미생물 계면활성제에 관한 연구(제2보);Pseudomonas sp. 13에 의한 Rhamnolipid의 생성 및 분리

  • Lee, Sun-Ju (Dept. of Industrial Chemistry, Chungbuk National University) ;
  • Nam, Ki-Dae (Dept. of Industrial Chemistry, Chungbuk National University) ;
  • Park, Heung-Jo (Dept. of Chemical Engineering, Chung ju Industrial Collegy)
  • 이선주 (충북대학교 공과대학 공업화학과) ;
  • 남기대 (충북대학교 공과대학 공업화학과) ;
  • 박흥조 (충주산업대학 화학공학과)
  • Published : 1992.11.30

Abstract

A microorganism, isolated from soil and designated Pseudomonas sp13, produced two kinds of rhamnolipid in the medium containing glucose as carbon source. There were both rhamnolipid contain L-rhamnose and ${\beta}$-hydroxydecanoic acid. Coumpound A and B elucted chloroform-methanol mixed solution of silicic acid column chromatography and recrystallized from a mixture of ether and n-hexane. Studies on the structure of these products reveled that compound A is L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoic acid and compound B is L-rhamnopyranosyl-L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoic acid.

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