Journal of Yeungnam Medical Science
- Volume 9 Issue 2
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- Pages.396-406
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- 1992
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- 2799-8010(eISSN)
Antimycotic Susceptibility Testing of Trichophyton Rubrum by Microculture Method
96-well microplate를 이용한 Trichophyton Rubrum의 항진균제 감수성검사
- Lee, Moo-Woong (Department of Dermatology College of Medicine, Yeungnam University) ;
- Kim, Jong-Chul (Department of Dermatology College of Medicine, Yeungnam University) ;
- Choi, Jong-Soo (Department of Dermatology College of Medicine, Yeungnam University) ;
- Kim, Ki-Hong (Department of Dermatology College of Medicine, Yeungnam University)
- 이무웅 (영남대학교 의과대학 피부과학교실) ;
- 김종철 (영남대학교 의과대학 피부과학교실) ;
- 최종수 (영남대학교 의과대학 피부과학교실) ;
- 김기홍 (영남대학교 의과대학 피부과학교실)
- Published : 1992.12.30
Abstract
Various susceptibility tests have been used to determine minimal inhibition concentration(MIC) of dermatophytes. They have limitations to apply practically because they need long time to determine MIC. Authors examined MIC of T. rubrum to ketoconazole and itraconazole using 96-well microplate and 24-well macroplate by method of Granade and Artis and tried to check the possibility of this method on clinical application. Nine strains of T. rubrum from patients with dermatophytosis were used. Evaluations of the factors affecting MIC were also tried. The results were as follows. 1. Effect of inoculation density on determination time and MIC : Determination of MIC were possible in 4th days after inoculation at higher inoculation density Caborbance 2.0, 1.0) compared to 6th days at lower inoculation density(absorbance 0.5, 0.25). 2. Effect of incubation temperature on MIC : When incubating at
저자들은 Granade와 Artis의 방법에 따라 96-well microplate와 24-well macroplate를 이 용하여 T. rubrum 9주를 대상으로 경구용 항진균제인 ketoconazole과 itraconazole에 대한 MIC를 측정하여 실제 임상사용 가능성을 알아보고 배양온도, 배양용기의 크기, 배지의 종류를 달리하여 MIC에 영향을 줄 수 있는 요소를 점검하여 다음과 같은 결론을 얻었다. 1. 96-well microplate를 사용하여
Keywords
- MIC(minimal inhibition concentration);
- Microculture;
- Trichophyton rubrum;
- Ketoconazole;
- Itraconazole