Analytical Science and Technology (분석과학)
- Volume 5 Issue 4
- /
- Pages.465-469
- /
- 1992
- /
- 1225-0163(pISSN)
- /
- 2288-8985(eISSN)
Assay of $\beta$ -Galactosidase Using High Performance Liquid Chromatography
고속액체크로마토그래피를 이용한 유당분해효소의 활성도 측정
- Shin, Myung Gon (Rice Utilization Research Center, Korea Food Research Institute) ;
- Chang, Pahn Shick (Department of Food Science and Technology, Seoul National Polytechnic University) ;
- Min, Bong Kee (Rice Utilization Research Center, Korea Food Research Institute) ;
- Kim, Sun Chang (Department of Food Science and Technology, Kyungnam University)
- Received : 1992.08.14
- Published : 1992.12.25
Abstract
An analytical procedure is presented for the quantitative determination of lactose, glucose, and galactose in the hydrolyzate of lactose by
유당분해효소의 활성도를 정량하기 위하여 고속액체크로마토그래피(HPLC)를 사용하는 방법을 확립하였다. Aminex HPX-87C 컬럼 및 RI 검출기를 사용하여 유당, 글루코스 및 갈락토스를 12분 이내에 정량적으로 분석할 수 있었다. 컬럼 온도는