The distribution of $^{14}C$-warfarin and the purification of hepatic microsome induced isozymes with coumarin

$^{14}C$-warfarin의 분포 및 쿠마린 유도체류에 의하여 간에서 유도된 동위효소의 정제

  • Park, Sung-Woo (Chemical Analysis Div., the National Institute of Scientific Investigation) ;
  • Kim, Eun-Ho (Chemical Analysis Div., the National Institute of Scientific Investigation) ;
  • Min, Ji-Sook (Chemical Analysis Div., the National Institute of Scientific Investigation) ;
  • You, Jae-Hoon (Chemical Analysis Div., the National Institute of Scientific Investigation) ;
  • Lee, Hee-Sung (Department of Biochemistry, Chung-Ang University) ;
  • Seo, Bae-Seck (Department of Chemistry, Catholic University Medical College) ;
  • Han, Wan-Soo (Department of Chemistry, Mok-Won College)
  • 박성우 (국립과학수사연구소 화학분석과) ;
  • 김은호 (국립과학수사연구소 화학분석과) ;
  • 민지숙 (국립과학수사연구소 화학분석과) ;
  • 유재훈 (국립과학수사연구소 화학분석과) ;
  • 이희성 (중앙 대학교 의과대학 생화학교실) ;
  • 서배석 (카톨릭의과대학 화학과) ;
  • 한완수 (목원대학 화학과)
  • Received : 1992.03.09
  • Published : 1992.03.25

Abstract

The $^{14}C$-warfarin used as rodenticids was identified from various organs of sprague dawley with scintillation counter. And the cytochrome p-450 which was induced by coumarin derivatives was identified with electrophoresis. The distributions of $^{14}C$-warfarin after $14.8{\mu}Ci/kg$ oral application at each organ was as follows; urine-7.5%, blood-0.44%, feces-0.9%, liver-0.66%, lung-0.86%, kidney-0.8%, heart-0.43% and spreen-0.33% after 24hrs. The cytochrome p-450 was purified by Octyl Sepharose CL-4B hydrophobic chromatography and isozymes were 50.8 Kd in control group, 53.3 Kd and 55.2 Kd in coumarin pretreated group and 50.8 Kd, 54.6 Kd and 57.7 Kd in warfarin pretreated group.

$^{14}C$-warfarin을 흰쥐에 경구투여 후 4시간 경과시 혈액에서 최고치를 나타내었으며, coumarin과 그 유도체인 warfarin에 의한 간조직 microsome 내의 Cyt. p-450은 이들 화합물에 의해 microsomal electron transport system이 증가됨과 Cyt. p-450의 isozyme들이 유도됨을 확인할 수 있었다.

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