Toxicological Research
- Volume 8 Issue 2
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- Pages.191-203
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- 1992
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
Immunosuppressive Effects of Safrole in BALB/c Mice
- Kim, Byung-Sam (Department of Biology, University of Ulsan) ;
- Jeong, Tae-Cheon (Department of Life Science, Korea Advanced Institute of Science and Technology) ;
- Choe, Suck-Young (Department of Food and Nutrition, University of Ulsan) ;
- Yang, Kyu-Hwan (Department of Life Science, Korea Advanced Institute of Science and Technology)
- Published : 1992.12.01
Abstract
The immunosuppressive effects of safrole were studied in female BALB/c mouse. Mice were given 100,200and 400mg safrole/kg daily for 14days and evaluated on day 15. The day 4 immunogloblin-M antibody response to T-dependent antigen, sheep red blood cells (SRBC) was inhibited dose-dependently in all doses studied. In vitro antibody response to polyclonal antigen, lipopolysaccharide (LPS) by spleen cell suspensions from safrole-treated mice were also significantly inhibited. When safrole was treated for 14days to mice, and mitogen-induced proliferation of splenocytes were assayed on day 15, there were significant suppression of responses to B-cell mitogen, LPS and T-cell mitogen concanavalin A(Con A) at a dose of 400mg safrole/kg. Direct addition of safrole on the splenocyte culture also produced a dose dependent suppression on in vitro antibody response to LPS, and mitogen-induced lymphoproliferatin at doses of 100,200,400 and 800
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