The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
권호 표지

Establishment of I-131, Tc-99m Labeling Methods to In-house Anti-CEA Antibodies and Evaluation of the Immunological Characteristics

국산 항 CEA 항체의 I-131, Tc-99m 표지법 확립 및 면역학적 특성 분석

  • Chung, June-Key (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Lee, Dong-Soo (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Lee, Myung-Chul (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Chung, Hong-Keun (Department of Biochemistry, Seoul National University College of Medicine) ;
  • Koh, Chang-Soon (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Hong, Mee-Kyoung (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Choi, Seok-Rye (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Seo, Il-Taek (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Chung, Jun-Ho (Department of Biochemistry, Dongguk University College of Medicine)
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 이동수 (서울대학교 의과대학 내과학교실) ;
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 정홍근 (서울대학교 의과대학 생화학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 홍미경 (서울대학교병원 핵의학과) ;
  • 최석례 (서울대학교병원 핵의학과) ;
  • 서일택 (서울대학교병원 핵의학과) ;
  • 정준호 (동국대학교 의과대학 생화학교실)
  • Published : 1992.12.31
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Abstract

Cancer cells have several tumor-associated antigens on the cell surfaces, and antibodies against these antigens have been developed by many investigators. Radiolabeled antibodies have been used as new methods to diagnose and treat malignant tumors. Especially anti-carcinoembryonic antigen (CEA) is the most popular antibody for these purposes. In this investigation, we tried to label $^{131}I$ and $^{99m}Tc $ to anti-CEA monoclonal antibodies which were developed in the Seoul National University College of Medicine. We found CEA-79 and CEA-92 antibodies had the better immunological characteristics among 8 anti-CEA monoclonal antibodies. And radioiodination of CEA-79 could be performed by chloramine-T method, while radioiodination of CEA-92 by iodogen method. To label these antibodies with $^{99m}Tc $, we used pretargeting transchelation as direct labeling method. At first, $^{99m}Tc $ was bound to glucaric acid, and monoclonal antibody was reduced by $\beta-mercaptoethanol$. When these were incubated together. $^{99m}Tc $ bound to glucarate was switched to monoclonal antibody because of higher affinity. We established conditions of several steps in this method. Anti-CEA monoclonal antibodies labeled with $^{131}I$ and $^{99m}Tc $ are expected to be used valuably in the detection and treatment of malignant tumors.

서울대학교 의과대학에서 개발한 항 CEA항체에 대한 방사성옥소 및 $^{99m}Tc$의 표지법을 개발하고 이런 표지항체의 면역학적 특성을 분석하였다. 1) 8가지의 항 CEA의 단세포군 항체중 CEA-79항체와 CEA-92항체가 가장 좋은 면역학적 특성을 보였다. 표지항체의 면역반응성은 60% 정도이었고, 항원/항체 친화상수는 $1\sim2\times10^9l/m$ 이었다. 2) CEA-79항체는 chloramine-T법으로, CEA-92항체는 iodogen 법으로 방사성옥소를 표지 하는 것이 가장 좋은 표지효율 및 면역 반응성을 보였다. 3) CEA-79항체는 0.5 mCi/mg에서부터 100mCi/mg까지 비방사능을 변화시켰을 때 표지효율이나 면역 반응성에 유의한 차이는 발견할 수 없었다. 4) Glucarate를 이용한 pretargeting transchelation법으로 CEA-79항체를 $^{99m}Tc$로 표지하는 조건을 확립하였다. 표지효율은 $60\sim70%$ 이었다. 본 실험을 통하여 얻은 적절한 조건의 $^{131}I$과 $^{99m}Tc$표지방법의 확립은 앞으로 방사면역신티그라피와 방사면역치료법에 유용하게 사용될 수 있을 것으로 기대된다.

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