Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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NMR Study of Effects of $MgCl_2$ on the Structural and Dynamical Properties of Yeast Phenylalanyl tRNA

  • Se Won Suh (Department of Chemistry, Seoul National University) ;
  • Byong Seok Choi (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Ki Hang Choi (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Jin Young Park (Department of Chemistry, Korea Advanced Institute of Science and Technology)
  • Published : 1992.10.20
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Abstract

Solvent exchange rates of selected protons were measured by NMR saturation recovery method for yeast $tRNA^{Phe}$, at temperature from 25 to $40^{\circ}C$, in the presence of 0.1 M NaCl and various low levels of added magnesium ion. The exchange rates in zero $Mg^{2+}$ concentration indicate early melting of acceptor stem, D stem, and tertiary structure. Addition of magnesium ion stabilizes the entire D stem more effectively than any other secondary or tertiary interactions.

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