Journal of Microbiology and Biotechnology
- Volume 2 Issue 4
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- Pages.268-272
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- 1992
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Specificity of Alcohol Dehydrogenase from Clostridium acetobutylicum ATCC 4259
- Kim, Byung-Hong (Michigan Biotechnology Institute) ;
- Zeikus, J.-Gregory (Michigan Biotechnology Institute)
- Published : 1992.12.01
Abstract
Alcohol dehydrogenase activity of Clostridium acetobutylicum ATCC 4259 was studied for its specificity against substrates in acidogenic and solventogenic cultures. The bacterium reduces propionate, valerate and caproate added to the medium to the corresponding alcohols. Acetaldehyde, propionaldehyde, butyraldhyde, pentanal, and hexanal were used as the substrates by alcohol dehydrogenase, and all were reduced to the corresponding alcohols with varying affinities and reaction velocities. Acetaldehyde showed the lowest affinity and lowest velocity while the other aldehydes showed similar