Production and Purification of Soluble Recombinant Human Lymphotoxin in Escherichia coli

  • Choi, Yoon-Ho (Department of Dairy Science, Sung Kyun Kwan University) ;
  • Na, Doe-Sun (College of Medicine, University of Ulsan) ;
  • Pan, Jae-Gu (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Park, Seung-Kiel (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Yoo, Hyang-Sook (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Kang, Kook-Hee (Department of Dairy Science, Sung Kyun Kwan University) ;
  • Sung, Moon-Hee (Genetic Engineering Research Institute, Korea Institute of Science and Technology)
  • Published : 1992.03.01

Abstract

Human lymphotoxin (LT) was produced in E. coli as a soluble protein. The level of recombinant human LT production was about 4% of the total souble proteins of E. coli extracts. Recominant human LT was purified to apparant homogeneity by a simple procedure utilizing FPLC on Mono Q and Mono S columns. The specific activity of the purified LT was $10\times10^7\;units/mg$.

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