한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제20권2호
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- Pages.136-142
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- 1992
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Bacillus stearothermophilus $\beta$ -D-Xylosidase 유전자의 크로닝 및 Escherichia coli에서의 발현
Molecular Cloning and Expression of Bacillus stearothermophilus $\beta$ -D-Xylosidase Gene in E. coli
초록
토양 분리균인 B.stearothermophilus chromosome의 유전자 은행으로부터 E.coli HB101 균주에
Bacillus stearothemophilus isolated from soil was identified to express multiple extracellular xylanases. Two HindIII restriction fragments of 5.4 and 6.4 kb from B. stearothermophilus genomic DNA were cloned into pBR322 to obtain recombinant plasmids pMG0l and pMG02, respectively, which enabled E. coli HBlOl cells to produce