Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Saccharomyces cevevisiae Mutants Deficient in (1$\rightarrow$3)-$\beta$-D-Glucan Synthase

베타-1,3-글루칸 생합성능이 손상된 Saccharomyces cerevisiae 돌연변이체의 선별 및 특성

  • Published : 1992.12.01
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Abstract

We have isolated conditional lethal mutants of Saccharomyces cerevisiae which are low in (1 ~3)-~-D-glucan synthase activity. These mutants were osmotic sensitive at nonpermissive temperature (37$^{\circ}$C) and showed a decreased level of alkali-insoluble cell wall glucan. The decrease in (1 ~3)-~-D-glucan synthase activity of the mutants appeared to be mainly due to the defect in catalytic component rather than in GTP-binding component.

본인 등은 베타-1,3-글루칸 합성효소능이 낮은 Saccharomyces cerevisiae의 조건성 돌연변이주들을 분리하여 그 특성을 조사하였다. 이 돌연변이주들은 비허용온도인 37'C에서 삼투감수성을 나타나며, 세포벽 성분 중 알칼리 비용해성 글루칸의 함량이 낮았다. 베타-1,3-글루칸의 합성효소능의 결함원인을 조사한 결과, 효모의 베타-1,3-글루칸 합성효소를 구성하는 촉매성분(cataytic component)과 GTP-결합성분 두가지 중 촉매 성분의 결함이 이 돌연변이주들의 베타-1,3-글루칸 합성효소능의 손상원인인 것으로 추정되었다.

Keywords

References

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