한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제20권6호
  • /
  • Pages.630-636
  • /
  • 1992
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

메탄올 자화성 효모의 분리, 동정 및 Aldehyde 생산

Isolation and Identification of Aldehyde Producing Methanol Utilizing Yeast

  • 윤병대 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 김희식 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 권태종 (건국대학교 미생물학과) ;
  • 양지원 (한국과학기술원 화학공학과) ;
  • 권기석 (건국대학교 미생물학과) ;
  • 이현선 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 안종석 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 민태익 (한국과학기술연구원 유전공학연구소 미생물공학실)
  • 발행 : 1992.12.01
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초록

토양으로부터 19균주의 메탄올 자화성 효모를 분리하였으며, 이들 중 높은 균체농도와 aldehyde 생산을 보인 균주를 선별 및 동정하여 Hansenula nonfermentant KYP-1으로 명명하였다. Aldehyde 생산은 메탄올 자화성 효모의 균체를 생촉매로 하는 resting cell system에서 행하였으며, 조사된 aldehyde 가운데 acetaldehyde의 생산량이 가장 높았다. 최대의 aldehyde 생산은 40시간 배양한 균체를 생촉매로 이용하였을 때 얻어졌다.

Hansenula nonfermentans KYP-l was selected and identified from 19 methanol utilizing yeasts isolated from soil samples by the enrichment culture technique. This strain showed a high cell concentration and a high aldehyde production. Aldehyde production was carried out in a resting cell system using methanol utilizing yeast as a biocatalyst. The molar yield of acetaldehyde was the highest among the aldehyde investigated, and the maximum amount of aldehyde was produced by cells obtained from a 40 hours' culture.

키워드

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