Ethanol Production Using Alginate Immobilized Cells of Zymomonas rnobilis

고정화 Zymomonas mobilis 균체로부터 에탄올 생산

  • 한면수 (중앙대학교 산업대학 식품가공학과) ;
  • 정동효 (중앙대학교 산업대학 식품가공학과)
  • Published : 1992.10.01

Abstract

The fermentation characteristics of ethanol production by the use of immobilized Zymomonas mobilis KCTC 1534 cells were investigated in terms of formation factors such as substrate and product concentration. In batch fermentation, the maximum values of specific ethanol productivity, specific substrate uptake rate, ethanol yield, and glucose conversion rate were $29.14g/{\ell}{\cdot}h$, $60.24g/{\ell}{\cdot}h$, 0.48g/g, and 98.4%, respectively, with 17% glucose medium, and its ethanol productivity was $2.91g/{\ell}{\cdot}h$ in the case of 25 hour fermentation time. Repeated batch fermentation was possible for 30 days with 2.24-$2.94g/{\ell}{\cdot}h$ ethanol productivity. In semicontinuous fermentation, the maximum ethanol productivity was shown to be $15.7g/{\ell}{\cdot}h$ at $0.36h^{-1}$ effective dilution rate with 17% glucose concentration. In this case, ethanol yield coefficient and glucose conversion rate were 0.39 g/g, 64.7%, respectively.

Zymomonas mobilis KCTC 1534 균체를 alginate에 고정화시킨 후 기질농도의 상관성으로 회분발효와 반연속발효에서 에탄올 생산을 시도하였다. 회분발효에서 17 glucose 농도를 발효시간 25시간에서 최대 에탄올 생산성 2.91g/l.h 얻었고 이경우 비에탄올 생산속도 $29.14g/{\ell}{\cdot}h$, 비기질 소모속도 $60.24g/{\ell}{\cdot}h$, 에탄올 수율 계수 0.48g/g, 기질전환율 98.4이었다. 생산배지의 전량 교환에 변수를 준 반복발효는 교환시간 20-24 시간에서 약 30일 동안 에탄올생산성이 2.24-$2.94g/{\ell}{\cdot}h$, 에탄올 수율계수 0.42-0.51g/g, 기질전환율 95.9-99.6%로 실행할 수 있었다. 반연속 발효의 경우 생산 배지의 교환 간격을 희석속도로 이용한 유효 희석속도 $0.36h^{-1}$에서 17% glucose 농도로 최대 에탄올생산성 $15.7g/{\ell}{\cdot}h$를 얻었고 이 경우 비 에탄올 생산속도 $156.9g/{\ell}{\cdot}h$ 비기질 소모속도 $396.0g/{\ell}{\cdot}h$, 에탄올 수율계수 0.39, 기질전환율 64.7% 이었다.

Keywords

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