한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제20권5호
  • /
  • Pages.565-573
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  • 1992
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

방선균 분리주 No. 1882-5로부터 Phorbol Ester에 의해 유도되는 K562 Cell의 소포형성을 억제하는 물질의 분리와 동정

Purification and Chemical Identification of the Inhibitor on Bleb Formation of K562 Cell Induced by Phorbol Ester from Actinornycetes Isolate No. 1882-5

  • 안종석 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 안순철 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 박문수 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 김보연 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 민태익 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 이현선 (한국과학기술연구원 유전공학연구소 미생물공학실) ;
  • 오원근 (한국과학기술연구원 유전공학연구소 미생물공학실)
  • 발행 : 1992.10.01
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초록

Phorbol ester에 의해 유도되는 K562 cell의 소포 형성을 저해하는 물질을 생산하는 방선균 분리주 No.1882-5를 토양으로부터 분리하였다. 분리주 No. 1882-5로부터 용매추출, Amberlite XAD-4, silica, Lobar low pressure LC를 거쳐 항진균 활성물질 MT-1882-I과 K562 cell의 소포형성을 억제하는 활성물질 MT 1882-II를 분리하였다. 이 물질들에 대한 이화학적 성질의 조사와 UV, $^1H-NMR$, $^13C-NMR$, mass spectrum의 분석에 의하여 MT 1882-II은 piericidin $A_{1}$($C_{25}H_{37}0_4N$, MW 415), MT 1882-II는 glucopiericidin A($C_{31}H_{47}0_9N$, MW 577)로 동정하였다.

We isolated Actinomycetes strain No. 1882-5, which produces the inhibitor on the bleb formation of K562 cell induced by phorbol ester, from soil sample. Through solvent extraction, Amberlite XAD-4, silica and Lobar low pressure LC, antifungal antibiotic MT 1882-1 and bleb forming inhibitor MT 1882-II were purified from strain No. 1882-5. MT 1882-1 was identified as piericidin $A_{1}$($C_{25}H_{37}0_4N$, M.W. 415) and MT 1882-11 as glucopiericidin A($C_{31}H_{47}0_9N$, M.W. 577) from the analysis of physico-chemical properties and UV, $^1H-NMR$, $^13C-NMR$, and mass spectra of these compounds.

키워드

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