Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 20 Issue 4
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- Pages.417-421
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- 1992
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Effects of Sucrose on Invertase Expression in Recombinant Saccharornyces cerevisiae
재조합 Saccharomyces cerevisiae에서 Invertase의 발현에 대한 Sucrose의 영향
Abstract
The expression pattern of the cloned SUC2 gene in recombinant Saccharomyces cerevisiae was investigated in a two-stage culture. The recombinant yeast grown in a glucose medium where the SUC2 gene was repressed was harvested and then resuspended in a sucrose medium to induce invertase expression. The maximum activity of 10 units was obtained in a medium containing 2
Sucrose에 의한 재조합 Saccharomyces cerevisiae의 invertase 생성 양성을 이단계 배양을 통하여 관찰하였다. 포도당 배지에서 SUC2 유전자의 발현이 억제된 상태에서 발현배지의 sucrose 농도가 2