Isolation and Characteris tics of Polyvinyl Alcohol Degrading Bacteria

폴리비닐 알콜 분해균주의 분리 및 특성

Two strains of polyvinyl alcohol (PVA) utilizing bacteria were isolated from the waste water and soil. These strains, G5Y and PW, were able to utilize PVA symbiotically as a carbon source, but could not utilize PVA separately. In the mixed culture of these strains, 0.5 percent of PVA was almost completely degraded in 3 days. Effect of degree of PVA polymerization on the its utilization was examined, and there was no remarkable difference among three kind of PVA (PVA 500, 1500, a d 2000). These bacteria were able to utilize PV,4 in the desizing waste water of factory as well as enrichment PVA medium. These strains, C5Y and PW, were identified as Pseudomonas cepucia and Pseudomonus pseudomallei, respectively, based on morpholofical and biological characteristics.

폐수중의 PVA를 생물학적 처리방법에 의한 제거를 위해 자연계로부터 PVA를 분해할 수 있는 세균을 분리하였으며, 그 중 균 성장육이 높고 PVA를 강력하게 분해, 자화할 수 있는 G5Y 균주와 PW 균주를 선별하여 동정 및 생육 특성을 조사하였다. 분리된 두 균주 G5Y와 PW는 혼합배양에 의해서만 PVa를 분해, 자화할 수 있으며, 두 균은 PVA 분해에 있어서 공생관계에 있음을 확인하였다. G5Y 균주와 PW 균주의 형태학적, 생화학적 등 제 특성에 따라 각각 Pseudomonas cepacia와 Pseudomonas pseudomallei로 동정되었다.