Purification and Properties of Trehalase from Rhizoctonia solani

Rhizoctonia solani가 생산하는 Trehalase의 정제 및 특성

  • 오태광 (한국과학기술연구원 유전공학연구소) ;
  • 서영배 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소)
  • Published : 1992.02.01

Abstract

Nonregulatory trehalase has been purified from mycelia of Rhztoctonia solani. a pathogen of rice sheath blight. Purification procedures involved sonification, gel filtration and fast protein liquid chromatography. Purity was confirmed by isoelectric focusing with silver staining. The purified trehalase was estimated to have a molecular weight of 54,000 and pI point of 5.1. Maximal activity was observed at pH 5.4 and temperature $45^{\circ}C$. The purified trehalase exhibited on apparent Km for trehalose of 3.11 mM and a Vmax of 105.3 $\mu mol min^{-1}\times mg^{-1}$. Validamycin, a commercial antibiotics of rice sheath blight, was a non-competitive inhibitor of Rhzzoctoniu solani trehalase.

벼 문고병원균인 Rhizoctonia solani의 균사체에서 비조절 trehalase을 초음파처리, gel filtration 및 Fast protein liquid chromatography를 통해서 분리하여 단일 단백질임을 확인하였다. 분리된 trehalase는 분자량이 54000, pI가 5.1인 단백질로 최대 역가가 pH 5.4, $45^{\circ}C$에서 나타났다. Trehalase 에 대한 Km치는 3.11mM, Vmax는 105.$\mu mol min^{-1}\times mg^{-1}$로 나타났고 벼 문고병 농용항생제인 validamycin은 trehalase에 대해서 non-competitive inhibiton을 하였다.

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