한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제20권1호
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- Pages.40-45
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- 1992
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Aspergillus niger의 Acidic Nucleotidase의 특성
Characterization of Acidic Nucleotidase from Aspergillus niger
초록
Aspergillus niger로부터 acidic nucleotidase를 Sepharose CL-6B gel 여과와 DEAE-Sephacel 이온교환수지를 이용하여 부분정제하였다. 5'-AMP 와 3'-AMP를 기질로 사용했을 경우에 효소의 최적 pH는 4.5, 그리고 최적온도는
Acidic nucleotidase from Asfiergilius nlger has been partially purified by Sepharose CL-6B gel filtration and DEAE-Sephacel ion exchange chromatography. The optimum pH and temperature for the enzyme reaction with 5'-AMP or 3'-AMP as a substrate were 4.5 and 55%, respectively. However, the optimum temperature became 70% when p-nitrophenyl phosphate was used as a substrate. The enzyme was stable at acidic pH. The enzyme activity was not affected by addition of various nucleotides, nucleosides and inorganic phosphates. Ferric, aluminium, vanadate and molybdate ions inhibited the enzyme activity dramatically. In kinetic studies,