Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 20 Issue 1
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- Pages.25-33
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- 1992
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
The Properties of Acetolactate Synthase Isozyme Produced by Serratia marcescens ATCC 254 19
Serratia marcescens ATCC 25419가 생산하는 Acetolactate Synthase Isozyme의 특성
Abstract
One acetolactate synthase isozyme which has Rf value of 0.83 on polyacrylamide gel electrophoresis was purified from Sewatia marcescens ATCC 25419 by ammonium sulfate fractionation, DEAE-Sephacel chromatography, Phenyl-Sepharose chromatography, Sephacryt S-400 gel filtration followed by native gel elution. The native molecular weight of the enzyme was determined to be 531,400 by gel filtration method, and SDS-polyacrylamide gel electrophoresis separated the native enzyme into two polypeptides having molecular sizes of 55,000 and 38,900 respectively. In kinetic parameters,
Serratia marcescens ATCC 25419를 질소원이 풍부한 BHI 배지에서 황산 암모늄 분별 침전을 시킨 후 DAEA-Sephacel chromatography, Phenyl-Sepharose hydrophobic chromatography, Sephacryl S-400 gel filtration, native gel elution을 거쳐 ALS isozyme Rf 0.83을 분리하였다. 분리한 ALS isozyme Rf 0.83의 native 형태는 gel filtration을 이용하여 분자량을 측정한 결과, 531,400이었고, SDS-PAGE를 수행한 결과 55,000의 large subunit와 38,900의 small subunit로 구성된 multimer임을 알 수 있었다.