Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 20 Issue 1
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- Pages.14-19
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- 1992
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Isolation and Identification of Exo-xylanase Producing Microorganism
Exo-xylanase 생산균의 분리 및 동정
Abstract
The xylanase producing microorganisms occurring on rotten woods were selectively isolated on the modified Czapek-Dox medium supplemented with 0.5% xylan as a sole carbon source. Among more than three-hundred isolates of xylanase producing microorganisms, only two bacterial isolates were turned out to be more potent xylanase producer than the reference strain of xylanase producer, Aureobaszdium pullulans NRRL Y-2311. The exo-xylanase producer, bacterial isolate No. 33 was identified as a strain of Pseudomonas sp. on the basis of morphological and biochemical characterizations as well as cellular fatty acid composition. Optima of pH and of temperature for enzyme reactions of xylanase were 5.5 and
부패한 나무, 퇴비, 제지공장의 폐지 및 폐수 등으로부터 분리한 300여 종류의 섬유소 분해균 중 xylanase 활성이 다른 균주에 비해 비교적 높았던 33번 균주를 균의 형태학적, 생화학적 특성과, 균체 지방산 조성에 의하여 Pseudomonas sp.로 동정하였다. Xylanase 활성의 최적 온도외 최적 pH는 각각