미생물학회지 (Korean Journal of Microbiology)
- 제30권6호
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- Pages.524-527
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- 1992
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
부영양화 수역에서의 Cyanophage 의 분리와 동정
Isolation and Identification of Cyanophage from Eutrophic Water
- Kim, Min (Research Center for Molecular Microbiology, Seoul National University) ;
- Choi, Yong-Keel (Research Center for Molecular Microbiology, Seoul National University)
- 발행 : 1992.12.01
초록
경기도 의왕시에 위치한 백운 저수지에서 분리된 cyanobacteria 인 Synechococcus sp. 로부터 한 종의 cyanophage 를 분리하여 ultrafiltration, differential centrifugation 과 sucrose density gradient centrifugation 을 이용하여 정제하였다. 분리된 cynophage 는 transmission electron microscope (TEM) 를 이용하여 100,000 배로 관찰한 결과 직경이 89 nm인 isometric head 와 tail 의 전체 길이가 111 nm 의 contractile tail 을 가진 phage 로서 Myoviridae 에 속함을 알 수 있었다. 생화학적 특징으로는 20~
Synechococcus sp. cyanophage was isolated from Baekwoon reservoir located in KyonggiDo. The cyanophage was purified by employing ultrafiltration. differential centrifugation. and sucrose density gradient centrifugation. Electron microscopic observation indicated that the sizes of its isometric head and contractile tail are 89 nm and] II nm. respectively. which means that the isolated cyanophage is included in the group. Myoviridae. The cyanophage maintained the stability of more than 50 percent from