미생물학회지 (Korean Journal of Microbiology)
- 제30권2호
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- Pages.108-112
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- 1992
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Expression and Secretion of Heterologous Protein in Yeast
- Kim, Moo-Kyum (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
- Song, Moo-Young (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
- Yu, Myeong-Hee (Genetic Engineering Research Institute, KAIST) ;
- Yu, Myeong-Hee (Genetic Engineering Research Institute, KAIST) ;
- Park, Hee-Moon (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
- Kim, Jinmi (Department of Microbiology, College of Natural Sciences, Chungnam National University)
- 발행 : 1992.04.01
초록
To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, .alpha.-1-antitrypsin (.alpha.-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the .alpha.1-AT expression vector pGAT6 which contained .alpha.-1-AT cDNA fused to GAL10-CYC1 promotor. The .alpha.-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was onfirmed both by the .alpha.-1-AT activity assay and by the immunoblot method using .alpha.-1-AT antibody. We also showed the secretion of .alpha.-1-AT into the culture media and into the periplasmic space by immunoblot.