Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Expression and Secretion of Heterologous Protein in Yeast

  • Kim, Moo-Kyum (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
  • Song, Moo-Young (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
  • Yu, Myeong-Hee (Genetic Engineering Research Institute, KAIST) ;
  • Yu, Myeong-Hee (Genetic Engineering Research Institute, KAIST) ;
  • Park, Hee-Moon (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
  • Kim, Jinmi (Department of Microbiology, College of Natural Sciences, Chungnam National University)
  • Published : 1992.04.01
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Abstract

To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, .alpha.-1-antitrypsin (.alpha.-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the .alpha.1-AT expression vector pGAT6 which contained .alpha.-1-AT cDNA fused to GAL10-CYC1 promotor. The .alpha.-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was onfirmed both by the .alpha.-1-AT activity assay and by the immunoblot method using .alpha.-1-AT antibody. We also showed the secretion of .alpha.-1-AT into the culture media and into the periplasmic space by immunoblot.

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