The Korean Journal of Zoology (한국동물학회지)
- Volume 35 Issue 4
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- Pages.466-473
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- 1992
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- 0440-2510(pISSN)
Purification and Characterization of Storage Protein-1 from Galleria mellonella
꿀벌부채명나방(Galleria mellonella L.)의 저장단백질-1(storage protein-1)의 정제 및 물리화학적 연구
Abstract
Storage protein-1 (SP-1) of Gallerio mellonella was identified in hemolvmph and fat body by electrophoresis. SP-1 was purified from hemolvmph by KBr density gradient ultracentrifugation , DEAE-cellulose (DE52) ion-exchange chromatography, and gel permeation chromatography (Sephadex G-200). Purity of SP-1 was confirmed by Non-SDS PAGE and electron microscope. SP-1 is 9.4 nm in diameter and regular octahedron in shape. SP-1 has isoelectric point of 5.7 and native molecular weight of 365 K dalton and is composed of one type of subunit with molecular weight of 82 K dalton. Ttiacylslvcerol and phospholipid were found to be maior lipid components in SP-1.