단일클론항체를 이용한 배양 계배 근원세포의 융합과 연관된 단백질의 확인

Identification of a Fusion-associated Protein in the Skeletal Myoblast Using Monoclonal Antibody

  • Kim, Chons-Rak (Department of Biolosy, College of Natural Sciences, Inje Uinuenity, Kimhae 621-749) ;
  • Won (Deportment of Biology, Department of Molecular Biology, College of Natural Sciences, Pusan National Uniuenity, Pusan 609-735. Korea)
  • 발행 : 1992.01.01

초록

The present study describes the production of monoclonal antibodies against cultured chick myoblast to pursue critical proteins in muscle cell fusion. Among a panel of monoclonal antibodies, three, Mll-3H 13, Mll-3Hl8 and Mll-3H35 were inhibited movblast fusion. A single 101-kDa antigen reactive with monoclonal antibody Mll-3H35 was detected by radioimmu-noprecipitation or by immunoblotting. During the course of myogenesis, the level of the protein remarkably decreased as the cells there differentiated. These results suggest that the protein platys a direct role in the process of myoblast fusion mechanism.

키워드