체외성숙 우난포란의 체외수정과 발달에 관한 연구 IV. Hyaluronic acid와 Chondroitin sulfate 및 Heparin이 우난포란의 체외수정에 미치는 영향

Studies on In Vitro Fertilization and Development of Bovine Follicular Oocytes Matured In Vitro IV. Effect of Hyaluronic acid, Chondroitin Sulfate, and Heparin on In Vitro Fertilization of Bovine Follicular Oocytes

These experiments were undertaken to investigate the rate of in vitro fertilization of bovine follicular oocytes treated with glycosaminoglycans(GAGs). Bovine follicular oocytes were obtained from the ovary of slaughtered animal and matured in media containing the various concentrations of hydluronic acid, chondroitin sulfate or heparin for 26 hours. Epididymal spermatozoa were capacitated and insemination was made by introducing about 10~15 matured oocytes into the suspension of spermatozoa. Six hour after insemination the eggs were transferred to TCM-199 supplemented with FCS(10%) and then examined the embryo development. After in vitro insemination, percentages of ova fertilized were 61.3 or 48.3%, respectively, for the cumulus intact or removed in the percentages of GAGs. However, in case of cumulus-free oocytes treated with GAGs, the fertilization rates were 58.8, 62.1, 58.8, and 61.8%, respectively, showing significant effect compared to 48.3% in cumulus-free oocytes. Our findings suggest that cnondroitin sulfate and heparin are superior to hyaluronic acid in the fertilizatin and pronuclear formation of bovine oocytes.