한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제16권1호
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- Pages.1-6
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- 1992
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- 1226-5284(pISSN)
체외성숙 우난포란의 체외수정과 발달에 관한 연구 IV. 난구세포의 생화학적 특성 검토
Studies on In Vitro Fertilization and Development of Bovine Follicular Oocytes Matured in Vitro
초록
These experiments were undertaken as a basic study to understand the role of cumulus cell on in vitro maturation and fertilization process with identifying the cumulus cell-secreted proteins. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and fast protein liquid chromatography(FPLC), the proteins of cumulus cells were identified. The results obtained in these experiments were summarized as follows ; 1. When the proteins secreted from cultured cell for 30 hours were separated by SDS-PAGE, there were a major band (>94,000) and other minor 2 bands with molecular weight ranging 30,000∼67,000 dalton. 2. In addition, the fractionations of these proteins by FPLC were idirectly shown that three bands were hyaluronic acid, chondroitin sulfate, and heparin.
키워드