Infertility of Transgenic Mice Experssing Human Growth Hormone Gene

사람 성장호르몬 유전자를 발현하는 형질전환생쥐의 불임성

  • 한용만 (한국과학기술연구원 유전공학연구소) ;
  • 강만종 (한국과학기술연구원 유전공학연구소) ;
  • 이철상 (한국과학기술연구원 유전공학연구소) ;
  • 유대열 (한국과학기술연구원 유전공학연구소) ;
  • 이경광 (한국과학기술연구원 유전공학연구소)
  • Published : 1992.09.01

Abstract

Many transgenic mice expressing human growth hormone gene were infertile. To investigate the infertility of these transfenic mice, it was looked into the estrus cycle and sexual behaviour and also tested through in vitro fertilization whether the germ cells of these mice normal or not. The infertile female transgenic mice were mated to the fertile males of ICR strain, but in almost all of them the vaginal plugs were not detected and their estrus cycles by vaginal smear were almost irregular which kept up estrus or diestrus stage. Many male transgenic mice did not have the ability of sexual behaviour. Therefore the viability of germ cells in infertile male transgenic mice was investigated by in vitro fertilization, but the sperm were normally fertilized with the eggs and the transgene of parent was passed on to the progeny. These results consequently suggest that the infertility of transgenic mice experssing human growth hormone gene may be due to the physiological activity of human growth hormone, not germ cells.

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