Archives of Pharmacal Research
- 제15권1호
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- Pages.30-34
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- 1992
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Microbial Transformation of Aniline to Acetaminophen
- Lee, Sang-Sup (College of Pharmacy, Seoul National University) ;
- Jin, Hyung-Jong (College of Pharmacy, Seoul National University) ;
- Son, Mi-Won (College of Pharmacy, Seoul National University)
- 발행 : 1992.03.01
초록
In order to obtain acetaminophen, a popular analgesic-antipyretic, through microbial p-hydroxylation and N-acetylation of aniline, various fungi and bacteria were secreened. Among them, Streptomyces species were chosen for strain improvement by the use of interspecific protoplast fusion technique. Two interspecific fused strains were developed between S. rimosus (N-cetylation function) and S. aureofaciens (p-hydroxylation function) and also between S. lividans and S. globisporus. For efficient protoplast fusion and cell wall regeneration, various conditions were examined. In a typical experiment of mixed S rimosus (