Journal of Ginseng Research
- 제16권3호
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- Pages.210-216
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- 1992
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- 1226-8453(pISSN)
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- 2093-4947(eISSN)
Benzo(a)Pyrene 유발 DNA 상해 및 복제 억제에 미치는 인삼사포닌의 영향
Effects of Ginseng Saponin on DNA Strand Breaks and Replication Inhibition by Benzo(a)Pyrene in CHO-Kl Cells
- Park, Jin-Kyu (Korea Ginseng & Tobacco Research Institute) ;
- Park, Ki-Hyun (Korea Ginseng & Tobacco Research Institute)
- 발행 : 1992.12.01
초록
The effect of saponin extracted from Panax grneng CA Meyer on DNA repair and replicative DNA synthesis were examined in CHO-Kl cells cotreated with benzo(a)pyrene and rat liver S-15 fraction. The DNA strand breaks inititated by benzo(a)pyrene metabolites were measured by alkaline election technique. The addition of ginseng saponin to the culture media resulted in decrease of benzo(a)pyrene-induced DNA strand breaks, and restored the suppressed-semiconservative-DNA-synthesis by the carcinogen. DNA repair synthesis in the damaged cells was also elevated by the ginseng treatment when the repairing activites were measured for the (3H)-thymidine incorporation into the carcinogen damaged cellular DNk Comparative analysis of DNA-adduces of benzo(a)pyrene metabortes in microsomes suggested that ginseng saponin treatment in rats reduced the formation of electrophilic metabolites of benzo (a)-pyrene in the rat liver microsomes.
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