Effects of Culture Condition on Callus Formation from Anther Culture of Petunia hybrida

Petunia hybrida의 약배양(約培養)으로부터 callus 형성(形成)에 미치는 배양조건(培養條件)의 영향(影響)

The study was conducted to get basic information for haploid production of Petunia hybrida through anther culture by investigating several factors, namely anther stage, culture medium, cold treatment, and genotypes. The results are summarized as follows; Four genotypes of Petunia hybrida were used in a study of anther culture. Plants of each genotype were grown in controlled environments at $20-30^{\circ}C$ with a 16h photoperiod. Equal numbers were harvested from each genotype. The anthers were taken from buds which had received the 14 days' cold treatment at $4^{\circ}C$. Anthers were dissected out aseptically and plated on 1/2 strength MS agar medium containing 5.0mg/${\ell}$ 6-Benzylaminopurine(BAP). Four weeks after culture, light green callus was formed. From these calli, plantlets were regenerated on MS medium containing 2.0mg/${\ell}$ 2-isopentenyl adenine(2ip) after 3 weeks.

본 연구는 Petunia hybrida $F_1$ hybrid를 약배양(葯培養)하여 반수체(半數體)를 얻을 목적으로 약(葯)의 치상시기(置床時期)와 배지(培地)의 종류(種類), 생장조절물질(生長調節物質)의 종류와 농도, 약(葯)의 전처리(前處理)와 품종(品種)에 따른 캘러스 형성률을 조사하였던 바 다음과 같은 결과를 얻었다. 약배양하기 전 0.8-1.2cm의 길이의 화뢰(花雷)가 달린 모본(母本)의 줄기를 $4^{\circ}C$에서 15일간 저온처리(低溫處理)하였다. 약을 채취하여 1/2 MS배지에 NAA 5.0mg/${\ell}$, BAP 0.5mg/${\ell}$를 첨가한 배지(sucrose 30g/${\ell}$, 2g/${\ell}$, pH 5.8)에서 배양 4주후 연녹색의 캘러스가 형성되었으며, 이들 캘러스로부터 배양 3주후 2ip 2.0mg/${\ell}$가 함유된 MS배지에서 식물체가 분화되었다.