Improvements of Protoplast Fusion Efficiency between Petunia hybrida and Nicotiana sandarae

Petunia hybrida와 Nicotiana sanderaer간(間) 원형질체(原形質體) 융합효율증진(融合效率增進)

This study was conducted to get basic information for factors affecting protoplast fusion between Petunia hybrida 'Titan red' and Nicotiana sanderae. The experiments such as fusogen, time of PEG treatment, temperature at fusion, $CaCl_2{\cdot}2H_2O$ concentration in fusion solution, and $CaCl_2{\cdot}2H_2O$ concentration and pH in eluting solution were carried out to increase the fusion efficiency. The results obtained were as follows; Fusion between P. hybrida and N. sanderae was accelerated when the mixture of the protoplasts was treated with 30% PEG 6,000 solution containing 5.5 mM $CaCl_2{\cdot}2H_2O$ for 10 minutes at $25^{\circ}C$, and subsequently eluted with a eluting solution containing 50 mM $CaCl_2{\cdot}2H_2O$ adjusted to pH 9.0.

Petunia hybrida와 Nicotiana sanderae의 원형질체(原形質體) 융합(融合)에 의한 체세포잡종식물(體細胞雜種植物)을 얻기 위하여 원형질체융합(原形質體融合)에 미치는 융합제(融合濟)의 종류, PEG의 처리시간(處理時間) 및 융합시(融合時) 온도(溫度), 융합용액(融合溶液)과 희석용액내(稀釋溶液內)의 $CaCl_2{\cdot}2H_2O$의 양(量) 및 희석용액(稀釋溶液)의 pH에 대해 실험(實驗)하였으며 그 결과(結果)는 다음과 같다. 두 종(種)의 원형질체(原形質體)를 $CaCl_2{\cdot}2H_2O$ 5.5mM이 함유(含有)된 PEG 6,000 30%용액(溶液)으로 $25^{\circ}C$에서 10분간 처리(處理)한 후 $CaCl_2{\cdot}2H_2O$ 50mM이 함유(含有)된 희석용액(稀釋溶液)의 pH를 9.0으로 조절(調節)한 용액(溶液)으로 희석(稀釋)시켰을 때 원형질체(原形質體) 융합율(融合率)이 가장 높았다.