Isolation of Rhodocyclus gelatinosus KUP-74 and its characteristic in ${\delta}-aminolevulinic$ acid production

Rhodocyclus gelatinosus KUP-74의 분리 및 ${\delta}-aminolevulinic$ acid 생산의 특성

A photosynthetic bacterium strain KUP-74 producing high amount of S-amino-levulinic acid(ALA) was isolated from soils, which was identified as Rhodocyclus gelatinosus. After 10 days cultivation under anaerobic-light condition at $30^{\circ}C$, 4 Klux and pH 6.8, 5 mg/l of ALA was formed extracellularly. ALA productions were increased up to 8 mg/l and 12 mg/l in cell cultivations either by the addition of 0.5% glycerol (v/v) or 10 mM of glycine and succinic acid, respectively, using Lascelles basal medium eliminated L-glutamic acid. By cultivation in the presence of 30 mM each D,L-glutamic acids and D,L-glutamines the yield of ALA showing a late induction phenomenon was reached the maximum value of 21 mg/l. Different culture times were needed to generate maximum ALA yields by the addition of initial precursors of $C_4$ and $C_5$ pathways in basal medium, as being 107 h and 262 h, respectively. 40 mg/l yield of ALA was observed by cell cultivation with the basal medium containing each 10 mM levulinic acid(LA) and glycine simultaneously.

토양으로부터 ${\delta}-aminolevulinic$ acid(ALA)의 생산성이 우수한 광합성세균 KUP-74f주를 분리하여 균학적 성질을 검토한 결과 이 균주는 Rhodocyclus gelatinosus로 동정되었다. 균의 배양은 $30^{\circ}C$, pH 6.8, 4Klux 텅스텐 광조도하에서 혐기적 정치배양하였으며, 배양 10일 후의 균체외 ALA 생산량은 5mg/l이었다. L-Glutamic acid를 제외한 Lascelles의 기본배지에 glycerol 0.5%(v/v)를 첨가 배양함으로써 ALA는 8 mg/l까지 증가하였으며, glycine과 succinic acid를 각 10 mM 첨가 배양함으로써 12 mg/l의 ALA가 생산되었다. 30 mM glutathione(redo-cod)의 첨가배양에 의하여 ALA 생산은 저하 되었으며, D,L-glutamic acid 및 D,L-glutamine에 의하여 ALA 생산이 late induction 되어 최고 21 mg/1에 이르렀다. ALA의 최대 생산량에 소요되는 배양시간은 ALA 생합성에 관한 $C_4$, $C_5$ pathways의 precursors 첨가시 각각 107 시간과 262시간이었다. 기본배지에 10 mM levulinic acid(LA)와 10 mM glycine을 동시에 첨가 배양함으로써 ALA 생산량은 40mg/l로 증가하였다.