Applied Biological Chemistry
- Volume 35 Issue 2
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- Pages.106-109
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- 1992
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Biosynthesis of artemisinin from 11,12-dihydroarteannuic acid
11,12-Dihydroarteannuic acid에서 artemisinin의 생합성
- Kim, Nam-Cheol (Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- Kim, Soo-Un (Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Seoul National University)
- Published : 1992.04.30
Abstract
To investigate the biosynthetic pathway leading to artemisinin, the putative precursors, arteannuic acid and 11,12-dihydroarteannuic acid, were incubated in a cell-free system. For the experiment with dihydroarteannuic acid, artemisinin was generated in tumor homogenate. These results showed that dihydroarteannuic acid could be converted to artemisinin enzymatically. However, the experimental condition failed to convert arteannuic acid into 11,12-dihydroarteannuic acid.
국화과의 일증인 개똥쑥(Artemisia annua L., 청호(靑蒿))에는 항말라리아 물질인 artemisinin이 함유되어 있다. 11,12-Dihydroarteanuic acid는 cell-free 방법으로 수행한 실험에서, Agrobacterium tumefaciens로 형질 전환된 crown gall의 homogenate와의 incubation에서 artemisinin으로 전환되었다. 그러나 arteannuic acid는 NADPH와 함께 incubation하였을 때 dihydro acid로 전환되지 않았다. 따라서 이 결과는 식물체의 조건에 따라서, dihydro acid가 자발적으로 뿐만 아니라, 생체내에서 효소 반응에 의해서도 artemisinin으로 전환될 수 있음을 나타낸다.
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