한국균학회지 (The Korean Journal of Mycology)

  • 제20권3호
  • /
  • Pages.234-239
  • /
  • 1992
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지

Virus 이병(罹病) 느타리버섯 (Pleurotus)으로부터 double-stranded RNA 의 분리(分離)

Molecular Analysis of double-stranded RNA in Abnormal Growing Oyster-Mushrooms, Pleurotos florida and P. ostreatus due to Virus Infection

  • 고승주 (농촌진흥청 농업유전공학연구소 분자유전과) ;
  • 박용환 (농촌진흥청 농업유전공학연구소 분자유전과) ;
  • 신관철 (충남대학교 농과대학 농생물학과) ;
  • Go, Seung-Joo (Division of Molecular Genetics, Agricultural Biotechnology Institute R. D. A.) ;
  • Park, Yong-Hwan (Division of Molecular Genetics, Agricultural Biotechnology Institute R. D. A.) ;
  • Shin, Gwan-Chull (Department of Agricultural Biology, Agricultural College, Chung Nam National University) ;
  • Wessels, Josep G.H. (Department of Plant Biology, University of Groningen)
  • 발행 : 1992.09.30
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초록

Virus 이병(罹病) 느타리버섯 (Pleurotus spp.)으로부터 이중나선(二重螺腺) ribo 핵산(核酸 )(ds RNA)을 분리(分離)하였다. Ds RNA 는 8100 base pairs(bp)의 큰 band 와 2170, 2120, 1980 및 1840 bp의 4개 작은 band로 이루어졌다. Ds RNA 분석법(分析法)으로 느타리버섯의 Virus 이병여부(罹病與否)를 조사(調査)한 결과(結果) 균사생장(菌絲生長)이 부진(不振)하고 세균성(細菌性) 갈색(褐色) 부패병(腐敗病) 등(等)에 이병(罹病)되고 이상자실체(異常子實體)를 형성(形成)하는 느타리버섯으로부터 Virus 이병(罹病)을 확인(確因)하였으나 건전(健全)버섯으로부터는 ds RNA를 분리(分離)하지 못하였다. 이 병(病)은 해외(海外)로부터 전래(傳來)한 것으로 보인다. Ds RNA 는 저농도염류액(底濃度鹽類液) (0.1XSSC)에서 RNase A 에 용해(鎔解)되었으며 $85^{\circ}C$ 에서 특성변화(特性變化)가 발생(發生)하였다.

The experiment was performed to find out the possibilities to detect virus infection in oyster mushrooms, Pleurotus species by analysis of doublestranded ribonucleic acid (ds RNA). Ds RNA segments were extracted from virus infected isolates which grew abnormally. But virus free isolates didn't show any ds RNA segments. The ds RNA was consisted of one large segment of 8100 base pairs (bp) and 4 smaller segments with 2170, 2120, 1980 and 1984 bp. Whereas, cell free virus particles showed only one larger ds RNA segment. The ds RNA was dissolved by RNase A in low salt, 0.1 M SSC and melted at $85^{\circ}C$. It was possible to use the ds RNA analysis for detecting virus infection directly from the host cells.

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