The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Studies on Light-Induced Mitochondrial ATPase in Pleurotus ostreatus -Effects of Organic Compounds-

느타리버섯 중의 Light-Induced Mitochondrial ATPase에 관한 연구 -유기물 효과-

  • Lee, Ho-Yeon (Department of Chemistry, College of Science, Dongguk University) ;
  • Min, Tai-Jin (Department of Chemistry, College of Science, Dongguk University)
  • 이호연 (동국대학교 이과대학 화학과) ;
  • 민태진 (동국대학교 이과대학 화학과)
  • Published : 1992.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Mitochondria in Pleurotus ostreatus were isolated and purified by stepped sucrose density gradient centrifugation, to compare the effects of organic compound on the activities of mitochondrial ATPase in Basidiomycotina with those in mammalian cell. The effects of N, N'-dicycio-hexylcarbodiimide (DCCD), carbonyl cyanide m-chlorophenylhydrazone (CCCP), sodium azide and aurovertin known as compounds to be related to electron transfer system in mitochondria were studied. A activity of mitochondrial ATPase was inhibited by 64%, 57% and 53% in the presence of 0.25 mM DCCD, 0.02 mM sodium azide and 1.5 $({\mu}g/mg\;of\;protein)$ aurovertin B, respectively. It was stimulated by 22% in the presence of 0.15 ${\mu}M$ CCCP.

Keywords