Establishment of a New Herbicides Screening Method Using Photoautotrophic Cultured Cell II. The Responses of Chlorophyllous Cells to Paraquat and Diuron in Tabacco

광독립영양세포(光獨立營養細胞)를 이용(利用)한 새로운 제초제(除草劑) 선발법(選拔法) 확립(確立) II. 담배의 녹화(綠化) 배양(培養) 세포의 Paraquat 및 Diuron 에 대한 반응(反應)

This study was conducted to determine the response of newly developed chlorophyllous cells against photosynthesis inhibitory herbicides in LS medium. Inhibition of the growth of the selected chlorophyllous cells in the LS medium containing sucrose 1%, NAA $10^{-5}$ M and BA $10^{-6}$ M under light condition increased as the concentrations of paraquat increased from $10^{-6}$ M to $10^{-4}$ M. The calli died in $10^{-4}$ M paraquat treatment and the inhibition of calli growth was greater when $CO_2$ was supplied. In the treatment of herbicide diuron, the inhibition of calli growth also increased as the concentrations of diuron increased from $10^{-6}$ M to $10^{-3}$ M and more inhibition was observed at 1% sucrose than 2% sucrose.

녹화된 cal1us의 제초제 paraquat와 diuron에 대한 반응을 요약하면 다음과 같다. LS 기본(基本) 배지(培地)에 sucrose 농도(濃度) 1.0%, NAA $10^{-5}$ M, BA $10^{-6}$ M을 첨가(添加)하여 paraquat를 처리(處理) 하였을때 농도(濃度)가 $10^{-6}$ M에 서 $10^{-4}$ M으로 증가(增加) 할수록 calli의 증식(增殖)이 억제(抑制)되고 엽록소(葉綠素) 함량(含量)이 감소(減少)되었다. $10^{-4}$ M에서는 완전(完全) 고사(枯死)하였으며 $CO_2$가 공급(供給)되는 조건(條件)에서 억제(抑制) 정도가 높았다. 제초제(除草劑) diuron 처리(處理) 시(時)에도 $10^{-6}$ M에서 $10^{-3}$M으로 농도(濃度)가 증가(增加) 할수록 callus 증식(增殖)이 억제(抑制)되었으며 sucrose농도(濃度) 2.0% 때 보다 1.0%에서 훨씬 높은 억제(抑制) 경향(傾向)을 보였다.