과배란유도시 난포기 LH의 생물학적 활성이 성숙난자의 수정율 및 난할율에 미치는 영향에 관한 연구

Effect of LH Bioactivity on Fertilization and Cleavage Rates of Mature Oocytes in Hyperstimulation Cycles for IVF-ET

  • 문신용 (서울대학교 의과대학 산부인과학교실) ;
  • 김석현 (서울대학교 의과대학 산부인과학교실) ;
  • 황태영 (서울대학교 의과대학 산부인과학교실) ;
  • 신창재 (서울대학교 의과대학 산부인과학교실) ;
  • 이진용 (서울대학교 의과대학 산부인과학교실) ;
  • 장윤석 (서울대학교 의과대학 산부인과학교실)
  • Moon, Shin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Kim, Seok-Hyun (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Hwang, Tae-Young (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Shin, Chang-Jae (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Lee, Jin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Chang, Yoon-Seok (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
  • 발행 : 1991.06.30

초록

To investigate the factors that affect the fertilization and cleavage rates of mature oocytes, 44 patients undergoing controlled ovarian hyperstimulation(COH) with FSH/hMG/hCG regimen for IVF - ET were analyzed. During follicular phase, serum LH levels were measured by radioimmunoassay and bioassay. Based on the mean follicular immunoactive LH(i-LH) and bioactive LH(b-LH) levels, patients were divided into 3 groups, respectively. There were no significant differences in basal serum FSH levels on menstrual cycle day 3, serum estradiol($E_2$) and progesterone ($P_4$) levels on the day of hCG administration, and the numbers of follicles aspirated and oocytes retrieved among groups. In relation to the mean follicular i-LH levels, the fertilization and cleavage rates of mature oocytes did not show a significant difference among groups. However, in groups with higher mean follicular b-LH levels, the fertilization and cleavage rates were reduced significantly. During late follicular phase, day-to-day variance in b-LH levels was not significant, but there was a significant difference among groups. There was no significant correlation between serum P. and b-LH levels. These data suggest that the fertilization and cleavage rates of mature oocytes are adversely affected by the raised mean follicular b-LH levels, and monitoring of serum b-LH levels is more useful in COH when compared with i-LH. It appears that the reduced rates are not due to the attenuated endogenous LH surge.

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