Effect of Guinea Pig Tracheal Epithelium on the Contraction of Rat Vascular Smooth Muscle

기니피그 기도상피세포가 백서의 혈관 평활근 수축에 미치는 영향

  • Kwon, O-Jung (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Yoo, Chul-Gyu (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Cho, Sang-Heon (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Park, In-Won (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Kim, Young-Whan (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Han, Sung-Koo (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Shim, Young-Soo (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Kim, Keon-Youl (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Han, Yong-Chol (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Seoh, Seok-Hyo (Department of Physiology, Seoul National University College of Medicine) ;
  • Kim, Ki-Whan (Department of Physiology, Seoul National University College of Medicine)
  • 권오정 (서울대학교 의과대학 내과학교실) ;
  • 유철규 (서울대학교 의과대학 내과학교실) ;
  • 조상헌 (서울대학교 의과대학 내과학교실) ;
  • 박인원 (서울대학교 의과대학 내과학교실) ;
  • 김영환 (서울대학교 의과대학 내과학교실) ;
  • 한성구 (서울대학교 의과대학 내과학교실) ;
  • 심영수 (서울대학교 의과대학 내과학교실) ;
  • 김건열 (서울대학교 의과대학 내과학교실) ;
  • 한용철 (서울대학교 의과대학 내과학교실) ;
  • 서석효 (서울대학교 의과대학 생리학교실) ;
  • 김기환 (서울대학교 의과대학 생리학교실)
  • Published : 1991.09.30

Abstract

It has been well known that the integrity of airway epithelium is important in developing of bronchial hyperreactivity or bronchial asthma. But the mechanisms underlying this nonspecific airway hyperresponsiveness are not yet determined. To evaluate the ability of guinea pig trachea to release an epithelium derived relaxing factor (EpDRF) which relax rat vascular smooth muscle, we performed the coaxial bioassay using guinea pig trachea and rat aorta. And to evaluate the nature of EpDRF we investigate the influence of methylene blue and indomethacin on the coaxial bioassay. Results were as follows. 1) Vascular smooth muscle mounted into the epithelium intact trachea which was precontracted with phenylephrine was relaxed by addition of histamine or acetylcholine. But vascular smooth muscle mounted into epithelium denuded trachea failed to be relaxed. 2) Epithelium dependent relaxation of vascular smooth muscle was not affected by pretreatment of methylene blue or indomethacin. These results strongly suggests that guinea pig tracheal epithelium releases EpDRF which is able to relax rat vascular smooth muscle. And EpDRF released by airway epithelium is not related to endothelium derived relaxing factor (EDRF) or cyclooxygenase products.

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Acknowledgement

Supported by : 서울대학교병원