고정화 미생물에 의한 뉴크레오타이드 생산

Production of Nucleotide by Immobilized Cell

5'-XMP의 효소적 전환에 의한 효율적인 5'-GMP의 생산을 위하여 동결 건조한 Bevibacterium ammoniagenes ATCC 19216을 K-carrageenan, polyacrylamide, Ca-alginate 및 agar등 4가지 담체에 고정화시켜 XMP aminase 효소원으로 사용하였다. 최적담체로서 선정된 $3\%$ K-carrageenan에 고정화한 균체를 이용하여 5'-GMP를 생산하였다. 고정화되지 않은 전환균주에 의한 5'-GMP의 최적 생산 조건은 $42^{\circ}C$, pH 7.0, 100mg/ml의 glucose, 8mg/ml의 $MgSO_4\cdot7H_2O,$ 5mg/ml의 POESA, 120mg/ml cell, 5mg/ml의 phytic acid으로 나타났으며, 이때 전환율은 $94.5\%$이었다. 고정화균체에 의한 5'-GMP의 최적생산 조건은 $37^{\circ}C$, pH 7.5, 50mg/ml의 glucose, 1mg/ml의 $KH_2PO_4,$ 10mg/ml 의 phytic acid, 60mg/ml의 고정화균체, 8mg/ml의 $MgSO_4\;\cdot\;7H_2O,$ POESA이며 이때 전환율은 $64.7\%$이었다.

The effective p.eduction of 5'-GMP(5'-Guanylic acid) by enzymatic conversion of 5'-XMP(5'-Xanthyic acid) was investigated. The Iyophilized Brevibacterium ammoniagenes ATCC 19216 which were used as the XHP aminase source, was immobilized by entrapping in K-carrageenan, agar, polyacrylamide or Ca-alginate. $3\%$ K-carrageenan was selected as the most suitable matrix. In the production of 5'-GMP using the free cells of 3. ammoniagenes ATCC 19216, the optimum conditions were $42^{\circ}C$, PH 7.0, 100mg/ml glucose, 120mg/ml cell ,8mg/ml $MgSO_4\cdot7H_2O$, 5mg/ml POESA, 5mg/ml phytic acid. Under the conditions, $94.5\%$ of 5'-GMP was converted within 8 hours. In the production of 5'-GMP using the immobilized whole cells of B. ammoniagenes ATCC 19216, the optimum conditions were $37^{\circ}C$, pH 7.5, 50mg/ml glucose, 1mg/ml $KH_2PO_4$, 10mg/ml phytic acid, 60mg/ml cell, 8mg/ml $MgSO_4\;\cdot\;7H_2O$, 5mg/ml POESA. Under the conditions, $64.7\%$ of 5'-GMP was converted within 40 hours.