The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
권호 표지

$^{99m}Tc-HMPAO$ Labelled WBC Scan in Experimental Abscess by Labelling Autologous Leukocytes With In-House-Synthesized HMPAO

국내 합성한 HMPAO 이용 $^{99m}Tc$ 표지 백혈구를 이용한 실험적 농양 스캔

  • Lee, Dong-Soo (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Shin, Hyung-Sik (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Ahn, Cu-Rie (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Chung, Jung-Key (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Myung-Chul (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Choi, Kang-Won (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Jung, Jae-Min (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Chung, Eun-Ju (Department of Nuclear Medicine, Seoul National University Hospital)
  • 이동수 (서울대학교 의과대학 내과학교실) ;
  • 신형식 (서울대학교 의과대학 내과학교실) ;
  • 안규리 (서울대학교 의과대학 내과학교실) ;
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 최강원 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 정재민 (서울대학교병원 핵의학과) ;
  • 정은주 (서울대학교병원 핵의학과)
  • Published : 1991.12.31
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Abstract

With HMPAO we have synthesized in our laboratory, we labelled $^{99m}Tc$ to canine leukocytes. Experimental abscess made by subcutaneous injection with Staphylococcus aureus was imaged with these $^{99m}Tc$ labelled leukocytes. Labelling efficiency of HMPAO with $^{99m}Tc$ was $66.2%{\pm}14.6%$ (N=9). Labelling efficiency of leukocytes with $^{99m}Tc-HMPAO$ was $54%{\pm}7.7%$ (N=7). Cell bound radioactivity in $^{99m}Tc-HMPAO$ labelled leukocytes was around 80% when these cells were incubated in plasma in vitro at $37^{\circ}C$ for 5 hours. In vivo cell bound activity was over 80% at 24 hours after injection. One day and four days after inoculation, uptake at the inflammatory focus was found with $^{99m}Tc$ labelled leukocytes. Uptake showed up in 4 hour image, and the uptake at the lesion was most prominent in 24 hour image. These findings show that in-house-synthesized HMPAO could be used for labelling leukocytes with $^{99m}Tc$, and that s$^{99m}Tc-HMPAO-labelled$ leukocytes were so stable and viable that inflammatory focus could be visualized with these $^{99m}Tc-labelled$ leukocytes.

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