한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제36권6호
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- Pages.506-512
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- 1991
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
면역측정법을 이용한 식물 홀몬의 분석 I. 옥신(IAA)에 대한 단크론 항체 생산
Analysis of Plant Hormones by Immunoassay I . Production of Monoclonal Antibodies to Indole- 3-acetic acid
초록
IAA에 대한 단크론 항체를 생산하고 이를 이용하여 생체중의 내생 IAA를 정량분석하기 위해 ELISA를 개발하고 본법을 사용하여 담배 종자 발아중 내생 IAA함량을 정량분석하였다. 그 결과는 1. IAA에 대한 단크론 항체 생산 세포주 3가지를 선발 작성하였으며 이 세포주로부터 생산되는 항체는 모두 IgG
Monoclonal antibodies (mAb) to indole-3-acetic acid (IAA) were produced and characterized. Spleen cells from mouse immunized with IAA coupled to bovine serum albumin were fused with SP2/0-Ag14 myeloma cells. Three clones secreted specific antibodies to IAA were established to hybridoma cell lines and designated WLI-G1, WLI-G3 and WLI-Ell. The antibodies produced were classified into IgG, types and revealed the high degree of specificity by cross-reaction in the IAA derivatives and its analogues. In the IAA-ELISA with mAb, the measuring range of the assay was 1-500 p mol, and Ka and binding capacity calculated from Scatchard plot were 6.7 X 10
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