보리의 수발아정도 및 탈곡방법이 종자활력 관련 형질에 미치는 영향

Effects of Sprouting Degree and Threshing Methods on Germination and Seedling Vigor in Barley

과맥에서 종자활력의 저하요인을 구명하고자 과맥 2계통(피, 과 isogenic line포함) 및 피맥 1품종의 종자의 수발아를 유도하여 손과 기계탈곡(700 rpm ) 한 종자를 이용하였다. 1. 수발아된 종자를 손 탈곡하여 배부분의 손상이 없어서 발아세, 발아율, TTC치은 수발아 정도에 따른 차이가 적었으나, 출아력은 수발아가 클수록 감소되었다. 2. 수발아된 종자는 기계탈곡시 배의 부분적인 손상이 관찰되었으며, 손 탈곡에 비해서 종자활력에 관계된 형질들이 고도로 유의적인 감소를 가져왔고 효소활력은 크게 증가되었다. 3 손 탈곡구에서 수발아 정도는 효소활력 ( r =0.9 31＊＊, 0.951＊＊) 및 발아력 ( r=0.46＊)과 정상관이 있었다.

In order for investigating the factors related to decrease of seedling vigor in the naked barley lines, two naked lines and a covered variety were sprouted with 20 mm rainfall in the natural condition, dried and the sprouted seeds were threshed by hand and small machine thresher, Chiyota. In the case of hand-threshing of the sprouted seeds, the germination rate index (GRI), germination rate, and TTC test value of the different varieties were not different due to the different sprouting degrees, but emergence rate index (ERI) became decreased with stronger sprouting damage. In comparison with hand-threshing, the embryos of sprouted seeds from machine threshing with 700 rpm were partially damaged, and the traits related to seedling vigor were decreased highly significantly. However, the diastatic activity was sharply increased by stronger sprouting and machine threshing. The sprouting degree in the hand-threshing plot was significantly correlated with the diastatic activity(r=0.931＊＊, 0.951＊＊) and GRI(r=0.461), respectively.