Journal of Microbiology and Biotechnology
- Volume 1 Issue 3
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- Pages.151-156
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- 1991
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Enhancement of Ethanol Tolerance of Lactose Assimilating Yeast Strain by Protoplast Fusion
- Ryu, Yeon-Woo (Department of Biotechnology, College of Engineering, Ajou University) ;
- Jang, Heang-Wook (Department of Biotechnology, College of Engineering, Ajou University) ;
- Lee, Haing-Sook (Department of Biotechnology, College of Engineering, Ajou University)
- Published : 1991.10.01
Abstract
In order to construct a yeast strain having high ethanol tolerance together with good lactose fermentation ability, the protoplast fusion using Saccharomyces cerevisiae STV 89 and Kluyveromyces fragilis CBS 397 was carried out. Auxotrophic mutants of K. fragilis were obtained as a selection marker by treatment of ethylmethane sulfonate. The best mutant for protoplast fusion was selected based on the capabilities of
Keywords
- Protoplast fusion;
- Kluyveromyces fragilis;
- enthanol tolerance;
- lactose fermentation;
- intracellular ethanol;
- unsaturated fatty acids