Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of Proteinaceous ${\beta}-Lactamase$ Inhibitor from the Culture Broth of Streptomyces sp. SMF-19

  • Kim, Myung-Kuk (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
  • Kang, Hee-Il (Department of Bioengineering, Yuhan Research Center) ;
  • Lee, Kye-Joon (Department of Microbiology, College of Natural Sciences, Seoul National University)
  • Published : 1991.08.01
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Abstract

The aim of this study is to elucidate characteristics of ${\beta}-lactamase$ inhibitor produced by Streptomyces sp. SMF-19 isolated from soil was found to produce proteinaceous extracellular ${\beta}-lactamase$ inhibitor. The ${\beta}-lactamase$ inhibitor was purified through ammonium sulfate fractionation, gel filtration, anion exchange chromatography and fast performace liquid chromatography. The molecular weight of the ${\beta}-lactamase$ inhibitor was estimated to be about 48,000 by SDS-PAGE. The mode of inhibition against penicillin G as a substrate was uncompetitive. The ${\beta}-lactamase$ inhibitor was stable in wide pH range but unstable at high temperature above $50^{\circ}C$.

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