Journal of Microbiology and Biotechnology

  • 제1권2호
  • /
  • Pages.85-89
  • /
  • 1991
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Purification and Characterization of Proteinaceous ${\beta}-Lactamase$ Inhibitor from the Culture Broth of Streptomyces sp. SMF-19

  • Kim, Myung-Kuk (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
  • Kang, Hee-Il (Department of Bioengineering, Yuhan Research Center) ;
  • Lee, Kye-Joon (Department of Microbiology, College of Natural Sciences, Seoul National University)
  • 발행 : 1991.08.01
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초록

The aim of this study is to elucidate characteristics of ${\beta}-lactamase$ inhibitor produced by Streptomyces sp. SMF-19 isolated from soil was found to produce proteinaceous extracellular ${\beta}-lactamase$ inhibitor. The ${\beta}-lactamase$ inhibitor was purified through ammonium sulfate fractionation, gel filtration, anion exchange chromatography and fast performace liquid chromatography. The molecular weight of the ${\beta}-lactamase$ inhibitor was estimated to be about 48,000 by SDS-PAGE. The mode of inhibition against penicillin G as a substrate was uncompetitive. The ${\beta}-lactamase$ inhibitor was stable in wide pH range but unstable at high temperature above $50^{\circ}C$.

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