Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Complete Nucleotide Sequence of Alkalophilic Bacillus sp. K-17 $\beta$-Xylosidase Gene

  • Chun, Hyo-Kon (Department of Food Science and Technology, Gyeongsang National University) ;
  • Ko, Hak-Ryong (Department of Food Science and Technology, Gyeongsang National University) ;
  • Kho, Yung-Hee (Genetic Engineering Research Institute, KIST)
  • Published : 1991.03.01
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Abstract

The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 $\beta$-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for $\beta$-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the nucleotide sequence of $\beta$-xylosidase gene, agreed with the result obtained by SDS-polyacrylamide gel electrophoresis of the purified enzyme (51, 000 dalton). The Shine-Dalgarno sequence, 5'-GAGGAGG-3', was found 8 bp upstream of the initiation codon ATG. The -10 sequence (TAAAAT) in the promoter region for $\beta$-xylosidase gene was similar to the consensus sequence for Bacillus subtilis RNA polymerase, whereas the -35 sequence (TCGATCA) different from all the known -35 regions in the promoter for Bacillus subtilis RNA polymerase.

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